Day 1 :
The Great Romagna Hub Laboratory, Italy
Keynote: How to facilitate and improve screening of Sexually-Transmitted Infections in women population
Time : 10:00-10:45
Vittorio Sambri is Director of the Great Romagna Hub Laboratory, Unit of Microbiology that is one of the largest public microbiology laboratory in Europe, and Associate Professor of Microbiology at the University of Bologna. His main research interest are spirochetes (in particular Treponema palidum infection) and emerging viral arthropod diseases. Recently his work has been focused on innovative moleculr diagnosis of sepsis and sexually transmited infections. His work resulted in 188 pblication in peer reviewed journal with an h-index of 37 amnd more than 4400 citations
Background: Sexually Transmitted Infections (STIs) are increasing worldwide. Innovative approaches are required to eliminate barriers to STIs testing such as home-based self-sampling for patients that are difficult to reach. Aim of this study was to evaluate performance of a new Home–based Self Vaginal FLOQSwabTM (HBSVF, COPAN Italia, Brescia) in combination with a commercially available real-time PCR assay, Anyplex™ II STI-7 (Seegene, Seoul, Korea) which detects seven pathogens in a single reaction (Chlamydia trachomatis CT, Neisseria gonorrhoeae NG, Trichomonas vaginalis TV, Mycoplasma hominis MH, Mycoplasma genitalium MG, Ureaplasma urealyticum UU, and Ureaplasma parvum UP). Methodology: 78 asymptomatic employees of a private industry (aged 18 to 45 years) were voluntarily enrolled to STIs screening. The subjects answered to a standardize anonymized questionnaire regarding the easy of use of self collection. The swab was collected in a domestic context by following the detailed “how to use it” instructions. After collection, swabs were shipped at room temperature to the laboratory in Pievesestina and processed within five weeks. The threshold cycle value (Ct) of a human genomic target (internal control, IC) and Ct of pathogens (CT, NG, TV, MH, MG, UU, UP) were taken as parameters to assess respectively, the efficiency of self-sampling and presence of any inhibitor effects, the stability of nucleic acids on dry swabs. Findings: no failure results were observed, the IC of all samples were amplified (average Ct 30). The real time PCR assay was able to identified 2/78 CT, 4/78 UU, 40/78 UP, 6/78 MH, 1/78 TV positive patients. No MG and NG positive patients were detected. Women reported self-collection with HBSVF easy and comfortable (100%). Conclusions: HBSVF device showed excellent recovery and stability of nucleic acid of STI pathogens up to 5 weeks at room temperature. The HBSVF is suitable for screening of STIs with real-time PCR assay.
China Medical University, Taiwan
Time : 11:05-11:50
Ying-Hen Hsieh is an applied mathematician and a professor at the College of Public Health, China Medical University in Taiwan. His primary research interests focus on mathematical and statistical modeling of infectious diseases as well as analysis of infectious disease epidemiology relating to public health policies.
Background/Objective Sexually transmitted disease is an ancient disease, although efficient treatment is available. Taiwan disease surveillance system disclosed that syphilis is the most common sexually transmitted disease. Among different age groups, the older population over 50 years of age had a relatively higher incidence. The study aims to focus the current syphilis epidemic in the ≧50 population in Taiwan.
Material and Methods Government syphilis surveillance data is used to infer the number of age over 50 years infected persons. Moreover, we utilize a discrete-time compartmental model to estimate important epidemiological parameters (i.e., infection rate, reporting rate) for two time periods, namely 2003-2006 and 2012-2014, in order to ascertain the recent trends of the epidemic.
Results Estimates for the first period (2003.9-2006.8) reveal that, new numbers entering ≧50-year old age group that are at risk of infection are 39.41% of 49-year-old population annually. The syphilis incidence of 50-plus age group is 1.6 cases per 100,000 persons. Estimate from the second period (2009.1-2011.12) indicate that, new numbers entering ≧50-year old age group that are at risk of infection are 1.26% of 49-year-old population annually. The syphilis incidence among 50-plus is 12.6 cases per 100,000 persons. Comparing the two period estimates, syphilis incidence surged sharply from 1.6 to 12.6 cases per 100, 000 persons, even though we have a decrease in newly entering syphilis at risk population (39.41 % to 1.26%). However, there has been a 3-fold increase in infection rate among the at-risk population.
Conclusions Syphilis incidence among age 50 and over population is increasing. Significant progress has been made on syphilis testing/surveillance program which led to substantial reduction in susceptible cases in general population. However, sexually transmitted disease prevention education program still need to be improved, to reduce infection in all adults, including the older aged population.